Dual color encoded Multiplex Beads
The spectral characteristics of the dyes used for the dual colour encoded system are:
|Color 1:||Excitation 420 – 480 nm||Color 2:||Excitation 515 – 540 nm|
|Emission 485 – 540 nm||Emission 535 – 570 nm|
Dual color encoded have been developed specifically for read-out using a fluorescence microscope in combination with a suitable pattern recognition software.
|Id||Mean Diameter||Color Labeling||Surface Modifications|
|105 12 011||11 µm||dual colour||3D-Carboxy AF|
|105 12 015||15 µm||dual colour||3D-Carboxy AF|
|105 31 011||11 µm||dual colour||Streptavidin|
|105 31 015||15 µm||dual colour||Streptavidin|
|105 32 011||11 µm||dual colour||Neutravidin|
|105 32 015||15 µm||dual colour||Neutravidin|
|105 33 011||11 µm||dual colour||3D-Alkyne|
|105 33 015||15 µm||dual colour||3D-Alkyne|
|105 34 011||11 µm||dual colour||3D-Azide|
|105 34 015||15 µm||dual colour||3D-Azide|
|105 35 011||11 µm||dual colour||Protein A/G|
|105 35 015||15 µm||dual colour||Protein A/G|
|105 36 011||11 µm||dual colour||3D-Aldehyde|
|105 36 015||15 µm||dual colour||3D-Aldehyde|
The standard packaging volume is 1.5 mL/population with a solids content of 0.5%. Please do not hesitate to contact us, if you are interested in a set of multiplex bead populations that is tailored to your specific application and/or read-out system.
Technology for dual-color bead encoding
The definition and differentiation between bead populations used in multiplex bead assays can be achieved using a combination of fluorescence emission wavelength (colour), fluorescence intensity and bead size. This encoding principle is illustrated in the image above.
PolyAn offers both dual-colour encoding and single-dye-encoding. Dual-colour encoded beads allow a narrower definition of the bead population, but have higher requirements regarding the read-out software.
By combining fluorescence encoding and different particle size populations up to 100 populations can be defined. Using size as well as fluorescence makes it easier to differentiate between populations. The overall system becomes more robust and the requirements with regards to the read-out system can be reduced.